| ORIGINAL ARTICLE |
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| Year : 2014 | Volume
: 9
| Issue : 4 | Page : 167-177 |
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Deflazacort alleviate pro-inflammatory cytokines expression, oxidative stress and histopathological alterations in collagen induced arthritis in Wistar rats
Neha, MdMeraj Ansari, Haider A Khan
Heavy Metal and Clinical Toxicology Laboratory, Department of Medical Elementology and Toxicology, Jamia, Hamdard (Hamdard University), Hamdard Nagar, New Delhi 110062, India
Correspondence Address:
Haider A Khan
Heavy Metal and Clinical Toxicology Laboratory, Department of Medical Elementology and Toxicology, Jamia, Hamdard (Hamdard University), Hamdard Nagar, New Delhi 110062
India
 Source of Support: None, Conflict of Interest: None  | Check |
DOI: 10.1016/j.injr.2014.06.007
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Objectives: Glucocorticoids are important and frequently used class of anti-inflammatory drugs. Deflazacort (DFZ) is a glucocorticoid and an oxazolone derivative of prednisolone. As it has high anti-inflammatory and immunosuppressive potency, we studied inhibitory effect of DFZ on mediators regulating the pro-inflammatory response and oxidative stress induced in arthritis. Methods: Female Wistar rats were immunized intradermally by collagen type II to induce collagen induced arthritis. Arthritic rats were treated with DFZ orally (6 mg kg-1 body weight) until 28 days after the onset of clinical symptoms of disease. The effect of DFZ treatment in the rats was monitored by clinical scoring, biochemical parameters, immu- nohistochemistry and histopathological evaluation.
Results: Deflazacort significantly decreased the level of articular elastase, nitric oxide and lipid peroxidation whereas significantly increased the activity of catalase, superoxide dismutase and glutathione. Deflazacort down-regulated expression of pro-inflammatory molecules like TNF-a and COX-2. Histopathological evaluation revealed significant reduc- tion of synovial hyperplasia and cellular infiltration in synovial membrane in DFZ treated group as compared to the diseased group.
Conclusions: This suggests that DFZ significantly down-regulates the expression of pro-in- flammatory molecules such as TNF-a and COX-2 and alleviates the oxidative stress which make it a viable therapeutic option for treatment of arthritis. |
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